Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: Genotypic association of rs4962153 genotype and ADAMTS13 antigen and activity. (A) The mean of ADAMTS13 antigen levels in participants with AA, AG and GG genotype of rs4962153 were 683.91, 568.02 and 327.26 ng/ml, respectively. ADAMTS13 antigen levels in participants with AA genotype were significantly reduced compared to both of AG and GG genotype ( P < 0.05 and < 0.001, respectively). Furthermore, ADAMTS13 antigen in participants with AG genotype was also significantly decreased when compared to GG genotype ( P < 0.05). (B) ADAMTS13 activity levels in participants with AA genotype (mean = 58.26%), were significantly reduced when compared to AG genotype (mean = 84.78%; P < 0.05) and GG genotype (mean = 95.53%; P < 0.001). The middle line referred mean of each individual genotype. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Activity Assay

Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: Inhibitory effect of MPs on ADAMTS13 activity. Non-filtered plasma samples presented ADAMTS13 activity 86.06%, which was significant lower than the filtered-plasma samples (mean = 104.03%). * P < 0.05.

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Activity Assay

Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: Effects of Febrile temperatures and Incubation time to ADAMTS13 activity*

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Incubation, Activity Assay

Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: ADAMTS13 activity was reduced in the incubation time and temperature-dependent manners. (A, B &C) Clear evidence of a time-dependent inhitition was observed either at 37°C, 38°C, or 39°C. * P < 0.05, ** P < 0.01, *** P < 0.001. (D) A temperature-dependent inhibition was observed. ADAMTS13 activity was significantly decreased at 38°C and 39°C incubation temperature, compared to 37°C for each incubation time except for 24 hr of 38°C compared to 37°C (NS = Not significant). All results represent mean ± SEM.

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Activity Assay, Incubation, Inhibition

Journal: Malaria Journal

Article Title: Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model

doi: 10.1186/1475-2875-13-3

Figure Lengend Snippet: Level of ADAMTS13 activity in plasma incubated in normal and febrile temperature

Article Snippet: Citrated plasma samples were collected from those participants and then the level of ADAMTS13 antigen and activity were performed using commercial kits (Quantikine Human ADAMTS13 ELISA kit; R&D Systems Inc., U.S.A, and Technozyme ADAMTS13 activity ELISA; Technoclone, Austria).

Techniques: Activity Assay, Incubation

Journal: ASAIO journal (American Society for Artificial Internal Organs : 1992)

Article Title: Benchtop von Willebrand Factor Testing: Comparison of Commercially Available VADs and Evaluation of Variables for a Standardized Test Method

doi: 10.1097/MAT.0000000000000849

Figure Lengend Snippet: Normal FFP, FFP+rhADAMTS13 (1 or 4μg/mL) and FFP+EDTA (10mM) were assayed for ADAMTS13 antigen level(A) and activity(B). The double solid line (=) denotes normal levels. Error bars are +/− one standard deviation.

Article Snippet: VWF and rhADAMTS13 antigen and activity levels were determined using ELISA-based kits: Quantikine ADAMTS13 antigen kits (ADAMTS13:Ag, R&D Systems), Technozym ADAMTS13 activity kits (ADAMTS13:Ac, DiaPharma, West Chester, OH), Technozym VWF collagen type I binding (VWF:CB), and Technozym VWF Antigen (VWF:Ag, DiaPharma).

Techniques: Activity Assay, Standard Deviation